Kit Contents

These contents may vary depending on which KIT is provided to the user.

• PCR thermal cycler
• UV transilluminator
• 1 high speed gel electrophoresis OR
• 2 low speed gel electrophoresis boxes (including combs and power supply)
• NB: there are alternate styles of gel boxes see equipment list
• 2 centrifuges (and power supply)
• 2 vortexes
• 3 green pipette pumps
• Sterile 5 ml pipettes (1 per working group)
• 6 boxes pipette tips (more if there is room in kit)
• 4 floating tube racks
• 2 - 4 beakers of sterile 1.5 ml tubes
• 10 microfuge tube racks
• ziplock bag of 0.2 thin walled tubes for PCR reactions
• 1 box Pasteur pipettes (they make cell spreaders with these)
• sterile toothpicks 4 containers
• micropipettors
• 2 waste bags
• Teacher's Instructions

Reagents

Sent at Room Temperature
Tube rack containing 5 aliquots of the following reagents
- STET buffer 1-2 ml each
- SOC 2 ml each
- Isopropanol 1-2 ml each
- 70% ethanol 1-2 ml each
- sterile water 1-2 ml each

PLUS

- 1 bottle of 10X TBE (200 ml, includes spare)
- 2 wide mouth jars with small amounts of 95% ethanol (for sterilizing cell spreaders), with MSDS labels on jars
- 2 microwaveable tubs with 0.8g of Agarose in each (plus 1 tub for each additional gel required)
- 2 tubes loading dye (1 - 1.5 ml each)
- 1 tube (24 ul) Sybr safe DNA gel stain ( for 2 gels includes spare)
- 12 MacConkey-Amp plates (10 for class plus 2 spare)
- 3 Agarose-Kan plates (teacher's use only)

Sent on Wet Ice (crushed ice in Ziplock bags)
Aliquots below all include spare amounts to compensate for minor spillage etc.
      - 12 tubes E.coli pellets
      - RNase (1 tube 25 ul)
      - PCR rxn mix (1 tube 100 ul)
      - PCR template plus primer (1 tube 100 ul)
      - 2X ligation butter (1 tube 60 ul)
      - T-vector (aka T-Easy) from ligation kit (6 ul diluted with 6 ul water to make up 12 ul)
      - T4 ligase (1 tube 12 ul)
      - 10X restriction buffer (comes with EcoR1 when we buy it) (1 tube 30 ul)
      - DNA marker (aka Ladder) (1 tube 15 ul)

Sent on Dry Ice
These are quantities PER CLASS and includes extras
      - competent cells (12 tubes)
      - lysozyme (1 tube 0.01 gm)
      - EcoR1 enzyme (1 tube 12 ul) for digest