LECTURES

Tuesday, Thursday 1:30 pm - 2:20 pm

Course content

I) Introduction: Proteomics and the analytical challenge

II) Bioanalytical Separations
1. Sample preparation
2. Pre-fractionation
3. Two-dimensional gel electrophoresis
4. Introduction to chromatography of biological molecules Reference book: "Modern HPLC for Practicing Scientists" by Michael W Dong, available electronically through the library catalogue
5. Reversed-phase and hydrophobic interaction chromatography
6. Ion-exchange chromatography
7. Size-exclusion chromatography
8. Multidimensional chromatography
9. Capillary electrophoresis
10. Protein-protein interactions

III) Mass Spectrometry in Biochemistry
1. Introduction
2. Overview and Background
3. MS Ionization
4. MS Fundamentals
a: Protein charge states
b: Resolution and mass accuracy
5. Instrumentation: TOF, Quadrupoles, hybrids
6. Understanding MS and MS/MS data
7. Quantitation
8. Post-translational modifications
9. Advanced Instrumentation: Orbitrap, FTICR

ANTIREQUISITES

PREREQUISITES

Biochemistry 3381A.

INSTRUCTORS

EVALUATION

Assignments (2 to 4), Midterm (1), Final Exam

If you have academic conflicts with the midterm test dates, inform Melita Hayes in the Biochemistry Office (Room M342, Medical Sciences Building) by email or in writing as early as possible. Written requests for alternate test times will be considered up to one week before the scheduled test. If you have a non-academic conflict with the midterm test dates, consult the Dean's office to determine if accomodation is warranted.

INSTRUCTIONAL INFORMATION

No texbook required. Lecture notes, handouts and research literature.

Scholastic Discipline
Statement on Academic Offences
University Policy for Plagiarism
Absences from course commitments
Support Services

Revised July 25, 2011 by Melita Hayes