Micromass® (now Waters®) M@LDI-LR bench-top Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer

Operating based on user walk-up access, high quality MALDI-MS data can be rapidly acquired for protein identification via peptide mass fingerprinting or intact protein analysis.  Operational training is provided upon requested, please contact the Facility for cost and schedule.  This instrument is funded by CFI (project entitled “Advanced Biotechnology Research Centre” awarded to Drs. Stan Dunn and Gary Shaw).

Bruker® Reflex IV Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer

The Bruker Reflex IV is a research grade MALDI TOF instrument equipped with a linear/reflectron mass analyzer and post-source decay (PSD) capability.  The built-in high resolution view finder allows users to search for “sweet-spot” in the sample, and thus enables ultra sensitive analysis at attomole level for peptides and femtomole level for proteins.  The extended mass range linear detector provides enhanced sensitivity up to m/z of 300,000. The mass resolution in reflectron mode is > 20,000.  This instrument was funded through a CFI New Opportunities grant to Dr. Ken Yeung.


Applied Biosystems® 4700 Proteomics Discovery System

The 4700 Proteomics Discovery System is based on a MALDI tandem time-of-flight mass spectrometer with TOF/TOF™ optics.  In additional to identification by peptide fingerprinting in the MS mode, this instrument can perform partial peptide sequencing of selected precusor ions after collision-induced dissocation in the MS/MS mode. This feature is extremely useful in improving the confidence in database searches, as well as identification of post-translational modifications.  The typically work flow of this system is optimized for proteomics research, particularly in delivering automated MS and MS/MS analyses at high sample throughput.  This instrument is funded by UWO Academic Development Fund to Dr. Ken Yeung and the Department of Biochemistry.