NMR Research
The Biomolecular NMR Facility is available to researchers who wish to conduct and analyze their
own NMR experiments or researches who simply wish to submit a sample and have us do the analysis
and interpretation for them. We are also available to discuss your research project with you and
help develop experiments that may address your biological questions.
Some of the capabilities of
the facility include:
• Characterization of small biological molecules
• Three dimensional structure determination of soluble proteins in solution
• Drug and receptor interaction
• Protein dynamics
• Mapping of protein-protein binding interfaces
• Probe protein conformational changes 
Laboratories working in the NMR Facility
Dr. Shaw's Laboratory
Dr. Choy's Laboratory
Dr. Li's Laboratory
Dr. Creuzenet's Laboratory
Recent Publications
• Rezvanpour A, Santamaria-Kisiel L, Shaw GS. (2011) The S100A10-annexin A2 complex provides a novel asymmetric platform for membrane repair. J Biol Chem. Sep 26. [Epub ahead of print]. Pubmed
• Dempsey BR, Shaw GS. (2011) Identification of Calcium-Independent and Calcium-Enhanced Binding between S100B and the Dopamine D2 Receptor. Biochem J. Oct 25;50(42):9056-65. Pubmed
• McCallum M, Shaw GS, Creuzenet C. (2011) Characterization of the dehydratase WcbK and the reductase WcaG involved in GDP-6-deoxy-manno-heptose biosynthesis in Campylobacter jejuni. Biochem J. Jun 28. [Epub ahead of print]. Pubmed
• Chaugule VK, Burchell L, Barber KR, Sidhu A, Leslie SJ, Shaw GS, Walden H. (2011) Autoregulation of Parkin activity through its ubiquitin-like domain. EMBO J. Jun 21;30(14):2853-67.
Pubmed
• Duncan KE, Dempsey BR, Killip LE, Adams J, Bailey ML, Lajoie GA, Litchfield DW, Brandl CJ, Shaw GS, Shilton BH. (2011) Discovery and characterization of a nonphosphorylated cyclic peptide inhibitor of the peptidylprolyl isomerase, Pin1. J Med Chem. Jun 9;54(11):3854-65. Pubmed
• Safadi SS, Barber KR, Shaw GS. (2011) Impact of autosomal recessive juvenile Parkinson's disease mutations on the structure and interactions of the parkin ubiquitin-like domain. Biochemistry. Apr 5;50(13):2603-10. Pubmed
• Spratt DE, Shaw GS. (2011) Association of the disordered C-terminus of CDC34 with a catalytically bound ubiquitin. J Mol Biol. Apr 1;407(3):425-38. Pubmed
• Santamaria-Kisiel L, Shaw GS. (2011) Identification of regions responsible for the open conformation of S100A10 using chimaeric S100A11-S100A10 proteins. Biochem J. Feb 15;434(1):37-48. Pubmed
• Mokhtarzada S, Yu C, Brickenden A, Choy WY. (2011) Structural characterization of partially disordered human Chibby: insights into its function in the Wnt-signaling pathway. Biochemistry. Feb 8;50(5):715-26. Pubmed
• Marlatt NM, Spratt DE, Shaw GS. (2010) Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins. Protein Expr Purif. Sep;73(1):58-64. Pubmed
• Safadi SS, Shaw GS. (2010) Differential interaction of the E3 ligase parkin with the proteasomal subunit S5a and the endocytic protein Eps15. J Biol Chem. Jan 8;285(2):1424-34. Pubmed
• Serniwka SA, Shaw GS. (2009) The Structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site. Biochemistry. Dec 29;48(51):12169-79. Pubmed
• Rezvanpour A, Phillips JM, Shaw GS. (2009) Design of high-affinity S100-target hybrid proteins. Protein Sci. Dec;18(12):2528-36. Pubmed
• Butty F, Aucoin M, Morrison L, Ho N, Shaw GS and Creuzenet C. (2009) Elucidating the Formation of 6-Deoxyheptose: Biochemical Characterization of the GDP-d-glycero-d-manno-heptose C6 Dehydratase, DmhA, and Its Associated C4 Reductase, DmhB. Biochemistry, 48(32):7764-7775. Pubmed
• Marlatt NM, Boys BL, Konermann L, Shaw GS. (2009) Formation of Monomeric S100B and S100A11 Proteins at Low Ionic Strength (dagger). Biochemistry, 48(9):1954-1963. Pubmed
• Li C, Schibli D, Li SS. (2009) The XLP syndrome protein SAP interacts with SH3 proteins to regulate T cell signaling and proliferation. Cellular Signalling 21:111-119. Pubmed
• Cooke CLM., Zhao L., Morton J., Shaw G., Arany E., Davidge ST., Regnault TRH. (2009) G-Protein Coupled Receptor-91 Is Differentially Altered in Kidneys from Male and Female Growth Restricted Rats: Implications for In Utero Programming of Hypertension. Reproductive Sciences, 16(3):324A.
• Shaw GS, Marlatt NM, Ferguson PL, Barber KR, Bottomley SP. (2008) Identification of a Dimeric Intermediate in the Unfolding Pathway for the Calcium-Binding Protein S100B. J Mol Biol., 382(4):1075-88. Pubmed
• Malik S, Revington M, Smith SP, Shaw GS. (2008) Analysis of the structure of human apo-S100B at low temperature indicates a unimodal conformational distribution is adopted by calcium-free proteins. Proteins., 73(1):28-42. Pubmed
• Ai X, Semesi A, Yee A, Arrowsmith CH, Choy WY, Li SS. (2008) The hypothetical protein Atu4866 from Agrobacterium tumefaciens adopts a streptavidin-like fold. Protein Sci., 17(1):154-8. Pubmed
• Serniwka SA, Shaw GS. (2008) 1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7. Biomol NMR Assign. Jun;2(1):21-3. Pubmed
• Yi S, Brickenden A, Choy WY. (2008) A new protocol for high-yield purification of recombinant human prothymosin alpha expressed in Escherichia coli for NMR studies. Protein Expr Purif. 57(1):1-8. Pubmed
• Safadi SS, Shaw GS. (2007) A disease state mutation unfolds the parkin ubiquitin-like domain. Biochemistry, 46(49):14162-9. Pubmed
• Yi S, Boys BL, Brickenden A, Konermann L, Choy WY. (2007) Effects of Zinc Binding on the Structure and Dynamics of the Intrinsically Disordered Protein Prothymosin alpha: Evidence for Metalation as an Entropic Switch. Biochemistry, 46(45):13120-30. Pubmed
•
Gall C, Xu H, Brickenden A, Ai X, Choy WY. (2007) The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity. Protein Sci., 16(11):2510-8. Pubmed
• Ai X., Li L., Semesi A., Yee A., Arrowsmith CH., Li SS., Choy WY. (2007) Hypothetical protein AF2241 from Archaeoglobus fulgidus adopts a cyclophilin-like fold. J. Biomol. NMR., 38(4):353-8. Pubmed
• Marlatt NM, Shaw GS. (2007) Amide exchange shows calcium-induced conformational changes are transmitted to the dimer interface of S100B. Biochemistry, 46(25):7478-87. Pubmed
• Beasley SA., Hristova VA., Shaw GS. (2007) Structure of the Parkin in-between-ring domain provides insights of E3-ligase dysfunction in autosomal recessive Pakinson's disease. Proc. Natl. Acad. Sci. U S A. 104(9):3095-100. Pubmed