FT-ICR Mass Spectrometer (IonSpec / Varian)

The QFT-12 is the newest mass spectrometer in the laboratory (installed December 2004). This new hybrid Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer has all the features of the traditional triple quadrupole with the superb performance characteristics and dissociation techniques (IRMPD, ECD, CID) of an FT-ICR. The QFT-12 is capable of obtaining mass spectra with high mass accuracy (< 1 ppm) with high resolving power in both broadband (resolving power ~ 100,000) or narrowband modes (resolving power >1,000,000).

This instrument is coupled to an Agilent nanoLC for LC-MS and LC-MS/MS protein characterization experiments, including identification and post-translational modification analysis. Powerful data-dependent LC-MS/MS analysis and "top-down" proteomics experiments can be performed on the QFT-12. This instrument can be used in positive and negative ion modes, and can be equipped with either a Micromass nanoESI source,  a prototype ChipLC (part of a collaboration with Agilent Laboratories), or a MassTech AP-MALDI source (Funded by ORDCF, 2004).

Q-Tof Global Ultima (Waters / Micromass)

The Q-Tof Global Ultima mass spectrometer (Micromass) is the  most sensitive Q-Tof mass spectrometer in BMSL. This instrument can be used in positive and negative ion modes using both electrospray and MALDI interfaces. Equipped with lockspray which allows the introduction of an internal standard without affecting ionization, this instrument is capable of obtaining accurate masses (<5 ppm) in V-mode (resolving power ~ 10,000) or W-mode (resolving power ~ 17,500).

MALDI-MS/MS capabilities make this a highly versatile instrument for the analysis of molecules that do not ionize efficiently with electrospray. This instrument is used routinely with a Waters nanoAcquity Ultra Performance LC for LC-MS and LC-MS/MS protein characterization experiments including identification and post-translational modification analysis (Funded by ORDCF, 2002).

Quattro Micro (Waters / Micromass)

The Quattro Micro triple quadrupole mass spectrometer is equipped with both electrospray and nanospray interfaces, as well as an Agilent 1100 HPLC system. It is used primarily for the routine MS analysis of small molecules, peptides, and proteins. It is capable of providing MRM, SIR, LC-MS, MS/MS, and precursor ion scanning data in both positive and negative ion modes to an upper m/z of 2000 (Funded by ORDCF, 2002).

Q-Tof Micro (Waters / Micromass)

The Q-Tof Micro mass spectrometer is a smaller version of the orthogonal acceleration (oa) Q-Tof mass spectrometers with a resolving power of 5000 and higher sensitivity than triple quadrupole mass spectrometers.

This instrument has both regular and nanoelectrospray interfaces and is coupled to a Waters CapLC for LC-MS and LC-MS/MS analysis. Currently, this instrument is used for routine peptide and protein analysis and protein identification (Funding CFI to Stan Dunn).

TOP